Experimental measurement of evolving AC properties during in vitro growth
The aims of these studies are to conduct in vitro growth protocols and measure articular cartilage (AC) structure-function relations. Newborn bovine patellofemoral AC explants are tested to determine mechanical (e.g. confined compression, unconfined compression, uniaxial tension, and torsional shear) and biochemical (i.e. contents of water, glycosaminoglycans, collagen type II, and collagen-specific pyridinoline crosslinks) properties before and after in vitro growth. In vitro growth protocols have included mechanically unstimulated growth in medium supplemented with fetal bovine serum (FBS), insulin growth factor (IGF), and transforming growth factor beta 1 (TGF-B1) and mechanically stimulated growth in a steady-state permeation bioreactor.
Typically, mechanical and biochemical properties evolve during, and are differentially regulated by, the different in vitro growth protocols. Statistical analysis is used to detect both significant changes in properties and significant structure-function relations (i.e. the relations between mechanical and biochemical properties). These results are then used as parameters for the cartilage growth mixture model and the cartilage growth finite element model.
Publications
- Ficklin T, Thomas G, Barthel JC, Thonar EJ, Masuda K, Asanbaeva A, Chen AC, Sah RL, Davol A, Klisch SM. Articular cartilage mechanical and biochemical property relations before and after in vitro growth. Journal of Biomechanics, 40:3607-3614, 2007.ABSTRACT PDF
- Stender ME, Dills KJ, Nelson LM, Williams GM, Stewart KM, Flores CR, Chen AC, Sah RL, Klisch SM. Differential regulation of articular cartilage compressive properties by IGF-I and TGF-b1 during in vitro growth. Transactions of the 2008 BMES Annual Fall Meeting, 2008.
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© 2008 Stephen M. Klisch | Mechanical Engineering