Rockfish DNA Lab Equipment
Checklist
Extraction
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scale
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weighing paper
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gloves
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forceps
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razor blades
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2" squares of
parafilm
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1.5 ml microcentrifuge
tubes
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tube holding racks (4)
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Buffer ATL
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Proteinase K
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DI water
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vortex
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heat block
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pipettes: (2) 200 µl,
(2) 20 µl
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pipette tips
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tip waste bins
Isolation
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ethanol
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vortex
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centrifuge
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heat block
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gloves
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1.5 ml microcentrifuge
tubes
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tube holding racks (8)
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QIAamp spin columns
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2 ml capless tubes
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Buffers: AL, AW1, AW2,
AE
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pipettes: (8) 200 µl,
(6) 1000 µl
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pipette tips
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tip waste bins
PCR
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ice
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gloves
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PCR tubes with beads
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Primer Solution
(CB3RF/Gludg/water)
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PCR thermocycler
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PCR tube racks (3)
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pipettes: (4) 20 µl
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pipette tips
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tip waste bins
Restriction Enzyme
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vortex
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heat block
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1.5 ml microcentrifuge
tubes
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Restriction Buffer
Solution (BSA, Buffer F, water)
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Sty1 enzyme
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(6) tube holding racks
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pipettes: (4) 20 µl,
(2) 2 µl
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pipette tips
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tip waste bins
Gel Electrophoresis
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scale
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weighing paper
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scoop
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Agarose
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TAE Buffer (3 liters)
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graduated cylinder
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50 ml Erlenmeyer flasks
(4)
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microwave
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gel tanks (4)
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power supplies (2)
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loading dye
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tube holding racks (4)
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pipettes: (4) 20 µl,
(4) 10 µl
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pipette tips
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tip waste bins
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EtBr tanks (2)
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water rinse tanks (2)
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safety glasses
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gloves
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spatulas
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Gel Doc setup with PC
and printer
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Ladder
PCR Cleanup
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Quiagen kit (QIAquick:
spin columns, buffers: PB, PE, EB)
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vortex
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centrifuge
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gloves
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1.5 ml microcentrifuge
tubes (2 jars)
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tube holding racks (6)
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2 ml capless tubes (2
jars)
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pipettes: (2) 20 µl, (6) 100 µl, (2) 1000 µl
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pipette tips: (1) red, (3) green, (1) blue
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tip waste bins
Cycle Sequence
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ice
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gloves
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PCR tubes
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Primers (1 µM CB3RF and
Gludg)
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PCR thermocycler
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PCR tube racks (8)
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pipettes: (4) 10
µl, (4) 2 µl
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pipette tips: (4) red
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tip waste bins
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Big Dye