UBL Protocol
for
Sterilization by Autoclave
Revised June
2002
1.н Place object(s) to be autoclaved in an autoclave tray (for easy carrying when you take them out!)
ннннннннннн
ннннннннннн § If autoclaving liquid media, place aluminum foil loosely over the top of the container.
ннннннннннн § If using a screw-on cap, loosen the cap before autoclaving.
2.н Place the autoclave tray in the autoclave (be careful, the walls and inside panel of the door is hot!)
3.н Close the autoclave door: push the door against the frame, then rotate clockwise to tighten.
ннннннннннн § Tighten the door only a little past hand tight (door becomes tighter as the run proceeds.)
.
4.н Turn the control handle to 'sterilize'.н Sterilize for 20-30 minutes, or if autoclaving liquid media, consult the following table:
|
Volume of Media н(per container; mL) |
Minimum Autoclaving Time (minutes) |
|
25 |
20 |
|
50 |
25 |
|
100 |
28 |
|
250 |
31 |
|
500 |
35 |
|
1000 |
40 |
|
2000 |
48 |
|
4000 |
63 |
* Minimum autoclaving time includes the time required for the volume to
reach sterilizing temperature (121 C) and 15 minutes at 121 C.
5.н Sign the usage log.
6.н After sterilizing for the desired amount of time, turn the control handle to 'fast exhaust' ('slow exhaust' for liquid media) and exhaust for ~10 minutes.
7.н After exhausting, turn the control handle to 'dry' for ~10 minutes (if you are rushed, this step may be skipped, but the contents may have condensation.)
8.н Turn the control handle to 'off'.
9.н Put on autoclave gloves, then open the door (turn counter-clockwise) and carefully remove the contents.
ннннннннннн § If the door won't open, just wait a few moments ё the pressure will subside.