Sequence of the intron housing pv92

o Primers designed to amplify parts of the intron that flank pv92 were used to determine if pv92 is present. If pv92 is present, the primers generate an 850 base pair PCR product. If pv92 is not present, the primers generate a 550 base pair PCR product.
o Cal Poly undergraduate students used cycle sequencing and UBL’s Applied Biosystems 377 DNA sequencer to sequence the 550 bp PCR product. This sequence is presented below

GCTGCTGCCTTTGAAAATGCTAGNAAAATAGTCCTGATCTATCTTTAATGCCTCAA

TGTTAGATATTCAGGTGACACCTAGAAAGGCACAATTGCTGATGACAGACTCTTG

GTGCTTTGAAATGGNAAAGATGGCAATATCTGAATTTTTATTCTCAATAACCTCCT

GGAGATTTGTAAGTTCCCTGCCAACTGCTTGTGTCTCCCCAACCCCCGCCATTGG

ACCAACAACCATGCCCCAGGCTCCACTCGAATCTACCCAGTCTCTGAGAAAATCG

TTACCAAGAAAGGCAAGCTACCAGAAGCCCCAACGCACAGNAGGGGTTCTGGAA

TAAATCTCCCCGTTCTCAACTCCTGTGTGTTTAGAGTGTGTTTAGAGAGGGAATTC

TTTCTTCTTGTAAGCCTCAAGTAACAGAGACAACCTTACTGAAATGTATCTGTGTG

ACTTTCTCTTCAATTTTCCAGGTAA