This page contains procedures & protocols commonly used in the UBL lab, including manufacturers' official protocols where applicable.

Alu Polymorphism (Using Blast) used by all students in bio 111,115,151

• Alu Sequence
• Alu Procedure and finding Alu Gene
• Finding Genes using BLAST
• Download Instructions for BLAST [alublast.doc]
• Download Instructions for Alu Procedure [polymorphism.doc]

Agarose Gel Electrophoresis

• UBL Procotol - Small Gel Box
• UBL Protocol - Large Gel Box
• Agarose Percent Calculator
• UBL Protocol - Using the Gel Doc

DNA Extraction - Rockfish used in Bio 427

• Rockfish Brief
• Rockfish Protocol
• Rockfish Checklist

DNA Extraction - Cheek Cells (Alu Polymorphism)

• UBL Checklist Protocol
• Qiagen Protocol [PDF]

DNA Extraction - Plant Tissue - LN2/Qiagen

• UBL Protocol - LN2 Tissue Prep
• Qiagen Protocol [PDF]

DNA Extraction - Plant Tissue - FastPrep

• UBL Checklist Protocol
• BIO101 Protocol [PDF]

DNA Extraction - Plasmid - MoBio

• UBL Checklist Protocol
• MoBio Protocol [DOC]

DNA Quantificaion - By Spectrophotometry

• UBL Protocol

DNA Sequencing with ABI 377 Sequencer used in bio BIO 375 and CHEM 375

• UBL Protocol - Instrument Setup
• UBL Checklist Protoco - Instrument Setup
• ABI Instrument User's Guide [PDF]
• ABI BigDye 3 Sample Preparation Guide [PDF]
• ABI BigDye Chemistry Guide [PDF]
• ABI Sequencing Analysis Manual [PDF]

Sterilization - By Autoclave

UBL Protocol