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Metabolism and population dynamics in monarch butterflies by Nicole van Aken and Tim Bozarth

Students are investigating the DNA sequence in the mitochondrial control region in monarch butterflies. This is part of a larger project examining various issues regarding metabolism and population dynamics in monarch butterflies. There are many unanswered questions about the local monarch population which overwinters on the Central Coast, including the relationship between this population and others in California as well as those east of the Rocky Mountains. Mitochondrial DNA has been used to answer questions about population structure and relationships in a variety of other organisms. As a first step, Nicole and Tim are using PCR to obtain a section of the mitochondrial control region, and then determining the DNA sequence of this region. They will perform this analysis on a sample of the local butterflies to determine how much sequence variation exists in this population and identify specific haplotypes; future students may then compare these sequences to those from other monarch populations.

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Last Update: April 15, 2004


Umdergraduate Biotechnology Laboratory Biological Sciences Department
California Polytechnic State University
San Luis Obispo, Ca 93407-0401
805.756.2826
pjankay@calpoly.edu